Rapid Plasmid DNA Mini Kit
For extracting plasmid DNA up to 30 μg of molecular biology purity within 8 min.
Rapid Plasmid DNA Mini Kit
- Plasmid DNA can be prepared within 8 min.
- No need of phenol and chloroformextraction.
- The revolutionary innovation Buffer N8 provides a floating-free supernatant within 2 min.
- Spin columns can be used without equilibration solution.
- The special addition of neutralization indicators allows first-time users to obtain the best plasmid extraction results.
Price List
| Product No | Quantity | Price |
| 1005050 | 50 preps | contact us |
| 1005250 | 50 preps | contact us |
Introduction
Based on the column purification plasmid kit, the Buffer N8 solution is designed to extract up to 30 μg of high-purity plasmid DNA from 1-5 ml of bacterial culture within 8 min without changing the user’s plasmid extraction habits.
Specifications
- Applications: Sequencing, restriction digestion, ligation and transformation, in vitro transcription/translation, transfection of strong cells
- Plasmid DNA can be prepared within 8 min.
- No need of phenoland chloroform extraction.
- Starting volume: 1-5 ml bacterial culture.
- Binding capacity: Up to 30 μg.
- Instruments required: A high-speed centrifuge that with rotor for 2 ml tubes.
Experimental data
Figure 1: 2 min centrifugation of the fast plasmid kit
Figure 2: centrifugation for 10 min with a common plasmid kit
- C: Electrophoresis effect of empty plasmidpUC-19.
- 1: dimeric plasmid DNA. 2: nicked plasmid DNA.3: supercoiled plasmid DNA.
- A: Single digestion effect of empty vector pUC-19.
- B: Rendering of pUC-19 double digestion with insert
Specifications
