Plasmid DNA Mini Kit
For extracting plasmid DNA up to 30 μg of molecular biology purity grades.
Plasmid DNA Mini Kit
- Spin columns do not require equilibration solution.
- The special addition of neutralization indicators allows first-time users to obtain the best plasmid extraction results.
- The specially designed Buffer W1 wash solution is designed to more thoroughly remove a variety of residual proteins, including non-restriction enzymes, for a wide range of E. coli strains, including wild-type host bacteria.
- Applications: Sequencing, restriction digestion, ligation and transformation, in vitro transcription/translation, transfection of strong cells.
Price List
| Product No | Quantity | Price |
| 1001050 | 50 preps | contact us |
| 1002250 | 250 preps | contact us |
Introduction
Based on the traditional alkaline lysis method for extracting plasmids, combined with column purification technology, it is suitable for extracting up to 30 μg of high-purity plasmid DNA from 1-5 ml of bacterial culture.
Experimental data
Figure 1: electrophoresis plot of pUC-19 plasmid DNA extracted with the Simgen Plasmid DNA Mini Kit.
The plasmid DNA was digested with different restriction enzyme effects.
1: the undigested pUC-19 plasmid DNA, 2: the digestion of the restriction enzyme with only one digestion site on the pUC-19 plasmid DNA, 3-8: the digestion effect of the restriction enzyme with multiple digestion sites on the pUC-19 plasmid DNA
M: Simgen 1kb DNA Ladder, 1.0% TAE agarose gel.
Specifications
Resources
Thesis
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