Endo-free Plasmid DNA Midi Kit
For extracting plasmid DNA up to 500 μg transfection purity.
Endo-free Plasmid DNA Midi Kit
- The purity is close to that of plasmid DNA prepared by two cesium chloride superdensity gradient ultracentrifugation.
- The specially designed extraction technology removes all kinds of residual bacterial lipopolysaccharides more thoroughly.
- Binding capacity: up to 500 μg.
Price List
| Product No | Quantity | Price |
| 1016025 | 25 preps | contact us |
Introduction
Based on the alkaline lysis method of extraction of plasmids, combined with column purification technology, it is suitable for the extraction of 200-500 μg of high-purity plasmid DNA from 40-80 ml bacterial cultures.
Specification
- Starting Volume: 40-80 ml.
- Binding capacity: 500 μg
- Instruments required: A high-speed centrifuge with rotor for 50 ml tubes.
- Applications: Eukaryotic cell transfection, restriction digestion, ligation and transformation, in vitro transcription/translation sequencing, DNA structure and function analysis.
Experimental data
Figure1 ：electrophoresis plot of plasmid DNA extracted with Simgen Endo-free Plasmid DNA Midi Kit.
1: plasmid DNA. 2: EcoRⅠSingle digestion product. 1%TAE agarose gel. M：Simgen 1Kb DNA Ladder
Specifications
