Bacteria DNA Extraction Kit
For extracting total DNA from 1-5 ml of bacterial cultures.
Bacteria DNA Extraction Kit
- The application of patented technologyis more novel and faster.
- Noneed of proteinase K and RNase
- The specially designed protein precipitation step ensures that the spin columnis not blocked when purifying DNA from excess bacteria.
Price List
| Product No | Quantity | Price |
| 3302050 | 50 preps | contact us |
| 3302250 | 250 preps | contact us |
Introduction
The bacteria cell was destroyed by lysozyme, and then lysed by Buffer L1. The protein and cell debris in lysate were precipitated by Buffer L2, and the genomic DNA in the supernatant can be bound to the spin column after centrifugation. After washing with Buffer WA and Buffer WB to remove proteins and PCR inhibitors remaining on the membrane, genomic DNA is eluted with Buffer TE and can be used immediately for various molecular biology experiments.
Specifications
- Applications: PCR, RFLP analysis, Southern Blotting.
- Binding capacity: up to 20 μg total DNA.
- Sample: Gram-positive or negative bacteria cultured in liquid medium.
- Elution volume: 100–200 μl.
- Instruments required: A high-speed centrifuge with rotor for 2 ml tubes.
Experimental data
Figure 1: Electrophoresis plot of DNA extracted from 4 gram-negative samples (collected from the same E. coli culture) using the Simgen Bacteria DNA Extraction Kit.
Figure 2: Electrophoresis plot of DNA extracted from 6 gram-positive samples (collected from the same culture of Bacillus subtilis) using the Simgen Bacteria DNA Extraction Kit.
Marker：Simgen DL15000 Ladder，1.0%TAE agarose gel.
Specifications
