Stool DNA Extraction Kit
Suitable for extracting total DNA (including bacterial and human or animal genomic DNA, mitochondrial DNA, and possibly viral DNA) from 150-200 mg of fresh or frozen human or animal stool.
Stool DNA Extraction Kit
- Prepare total stool DNA within 40-50 min.
- Simultaneous extraction of human or animal intestinal exfoliated cell’s DNA, bacterial DNA, viral DNA and parasite DNA.
- No need of phenol chloroform and isopropanol.
- Silica technologyremoves PCR inhibitors from the stoolquickly.
Price List
| Product No | Quantity | Price |
| 4101050 | 50 preps | contact us |
| 4101250 | 250 preps | contact us |
Description
This kit has a strong lysis buffer to lyse the stool sample and release DNA. Then use the proteinase K to digest the supernatant. The inhibitors and degraded proteins in the stool were filtered out by column purification technology. The total DNA eluted directly with Buffer TE and is ready for use in various molecular biology experiments.
Specifications
Applications: PCR, infectious disease research & detection.
Samples: Fresh or frozen human or animal stool.
Starting sample: 150-200 mg.
Instruments required: A high-speed centrifuge with rotor for 2 ml centrifuge tubes.
Experimental data
Figure 1: Electrophoresis plot of stool DNA extracted with Simgen Stool DNA Extraction Kit.
1, 2, 3, 4：Genome DNA extracted from fresh stool. M：Simgen DL15000 Ladder
Figure 2: Amplification plot of HBV specific Primers.
Curve 1: DNA extracted with Simgen Stool DNA Extraction Kit from the stool of HBV carriers was used as a template to detect HBV (5 μl DNA added in a 50 μl amplification system).
Curve 2: DNA extracted by boiling method from the same stool of HBV carriers was used as a template to detect HBV (5 μl DNA added in a 50 μl amplification system).
Specifications
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Resources
Thesis
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- Li K, Cao R, Mo S, et al. Swine manure composting with compound microbial inoculants: Removal of antibiotic resistance genes and their associations with microbial community[J]. Frontiers in Microbiology, 2020, 11: 592592.
- Feng J, Wang L, Chen Y, et al. Effects of niacin on intestinal immunity, microbial community and intestinal barrier in weaned piglets during starvation[J]. International Immunopharmacology, 2021, 95: 107584.
- [PDF] Feng J, He D, Chen T, et al. Nicotinic Acid Upregulates Intestinal Antimicrobial Peptides via Manipulating the Histone Acetylation Modification to Enhance the Resistance of Escherichia Coli Infection in Weaned Piglets[J]. 2021.
- Zhen R, Liu C, Wei C, et al. Effect of different dosages of sodium butyrate and niacin on growth, faecal microbiota and Vitamin B metabolism in weaned piglets[J]. Journal of Applied Microbiology, 2022, 132(6): 4466-4475.
- Zhen R, Feng J, He D, et al. Effects of Niacin on Resistance to Enterotoxigenic Escherichia coli Infection in Weaned Piglets[J]. Frontiers in Nutrition, 2022, 9.