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Description

The body fluid sample is digested by proteinase K to release viral nucleic acid, and then supplemented by column purification technology to adsorb viral nucleic acid on the spin column. After washing the spin column with a wash buffer, the impurities and PCR inhibitors remaining on the spin column can be completely removed. Viral nucleic acids on the spin column can be eluted directly with Buffer TE and can be used directly in various molecular biology experiments.

Specifications

• Samples: body fluids (plasma, serum, urine, CSF, and cell culture supernatant).
• Sample amount: 200 μl body fluid sample.

Experimental date

Figure 1: Amplification plot of HBV DNA

Curve 1: Nucleic acids extracted in serum by boiling method (5 μl DNA added in a 50 μl amplification system).

Curve 2: Nucleic acids extracted from the same serum (200 μl) with Simgen Virus Nucleic Acid Extraction Kit (5 μl DNA added in a 50 μl amplification system).

Curve 3: Nucleic acids extracted from the same serum (200 μl) with Simgen Virus Nucleic Acid Extraction Kit (25 μl DNA added in a 50 μl amplification system). No significant inhibition effect was observed, but the CT value was reduced.

Specifications