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Blood DNA Extraction Mini Kit

Rapidly extract up to 20 μg total DNA from 400 μl anticoagulated whole blood in less than 15 min.

Blood DNA Extraction Mini Kit

Blood DNA Extraction Mini Kit

  • Prepare total blood DNA within15 min.
  • No need for prior separation and removal of red blood cells and proteinase K digestion.
  • Can be transported and stored at room temperature
  • Completely remove PCR inhibitors and usingup to 1/2the PCR system volume of DNA for template

Price List

Product No Quantity Price
3001050 50 preps contact us
3001250 250 preps contact us

Description

This kit eliminates the traditional proteinase K digestion mode and uses an innovative denaturation precipitation method to remove hemoglobin. Column purification technology can completely remove impurities and PCR inhibitors that remain on the spin column. Total DNA eluted with Buffer TE or water can be used immediately for a variety of molecular biology experiments.

Specifications

  • Application: PCR, RFLP, Southern blotting, Genotyping
  • Samples: fresh or frozen whole blood, hemolyzed whole blood, saliva, cell suspension, etc.
  • Spin column binding capacity: 20 μg total DNA
  • Sample amount: 200 – 400 μl whole blood
  • Instruments required: A high-speed centrifuge with rotor for 2 ml tubes.

Experimental data

Table 1: 6 different anticoagulation EDTA whole blood samples, DNA extraction efficiency of 400 μl blood samples measured (100 μl Buffer TE elution)

Figure 1: Genomic DNA electrophoresis from 6 different human blood samples extracted with the Simgen Whole Blood DNA Extraction Mini Kit.   (lanes 2 and 3, where the DNA bands of apoptotic cells are visible).

Figure 2: Extracted DNA from fresh whole blood (lane 1) and whole blood stored at 2-8°C (lanes 2 and 3, where the DNA bands of apoptotic cells are visible).

 

Resources

Instructions
Instructions

Thesis

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