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Description

This kit uses a strong lysate to lyse the blood and precipitate hemoglobin in one step. After centrifuging, add the supernatant to the spin column. DNA is bound to the spin column, and the residual protein and PCR inhibitors are filtered and removed, and the DNA is washed once by Buffer WB, and then eluted with Buffer TE, which can be used for various molecular biology experiments.

Features

• Easyto operate, specially spin columns,only need to filter the column once, wash once
• No use of Red Blood Cell Lysis Buffer
• No need for tedious steps such as proteinase K digestion and ethanol addition
• Three-in-onelysate functions: dissolving blood, releasing DNA, and precipitating hemoglobin

Experimental data

Table 1: DNA extracted from 300 μl and 600 μl of blood from the same normal human blood sample (one tube in duplicate) with the Rapid Blood DNA Extraction Mini Kit (elute DNA with 100 μl TE buffer)

Figure 1: The DNA form Table 1, the sample load was 5 μl for electrophoresis.

M：Simgen DL15000 Ladder

Figure 2: 18 μl of each of the DNA extracted in Table 1 was used as template (40 μl amplification system) for PCR amplification, and no inhibition effect was observed.

5: negative control. 6: positive control. M：Simgen DL2000 Ladder.

Human β-globin gene primer (Gene of interest approx. 1.3 kb).