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Animal Tissue DNA Extraction Kit

Suitable for extracting total DNA (including genomic DNA, mitochondrial DNA, and possibly viral DNA) from ≤25 mg fresh or frozen human or animal tissues.

Animal Tissue DNA Extraction Kit

Animal Tissue DNA Extraction Kit

  • Proteinase K digestion method
  • Suitable for various animal tissues: rat tail, rat ear, formalin-fixed tissue, and more etc.
  • Suitable for fluid samples: anticoagulated whole blood, saliva, cultured cell suspensions, etc.
  • Suitable for insects, gram-negative bacteria, or gram-positive bacteria.
  • No need of phenol chloroform and isopropanol.

Price List

Product No Quantity Price
3101050 50 preps contact us
3101250 250 preps contact us

Description

After the tissue sample is digested by proteinase K, the degraded protein is filtered out by column purification technology. Total DNA adsorbed on the spin column and washed by Buffer WA and Buffer WB, which can completely remove the impurities and PCR inhibitors remaining on the spin column. Total DNA eluted directly with Buffer TE or water can be ready for use in various molecular biology experiments.

Specifications

  • Application: PCR, RFLP analysis, Southern Blotting.
  • Column binding capacity: Up to 30 μg DNA.
  • Sample amount: ≤ 25 mg of fresh or frozen human or animal tissue.
  • Instruments required: A high-speed centrifuge with rotor for 2 ml tubes.

Experimental data

Figure 1: DNA Extracted from four mouse tissue samples with Simgen animal tissue DNA Extraction Kit.

1.0% TAE agarose gel electrophoresis. M: Lambda-Hind Ⅲ Ladder. 1: Heart tissue2: Liver tissue3: Lung tissue 4: Kidney tissue

Specifications

Resources

Instructions
Instructions

Thesis

  1. Zhang Z H, Zhao X J, Song Y Z, et al. Cloning and sequence analysis of SLC25A13 transcripts in human amniocytes[J]. Translational Pediatrics, 2012, 1(2): 85.
  2. Wei M, He L, Yang X, et al. The complete mitochondrial genome of Pseudobagrus albomarginatus Rendhal: sequencing and analysis[J]. Mitochondrial DNA, 2015, 26(4): 633-635.
  3. Li G, Xu J, Wang H, et al. Identification and analysis of the complete mitochondrial genome sequence of Eriocheir sinensis[J]. Mitochondrial DNA Part A, 2016, 27(6): 3923-3924.
  4. Zhou J H, Zhou Q Z, Lyu X M, et al. The expression of cysteine-rich secretory protein 2 (CRISP2) and its specific regulator miR-27b in the spermatozoa of patients with asthenozoospermia[J]. Biology of reproduction, 2015, 92(1): 28, 1-9.
  5. Liu Q N, Zhang H B, Jiang S H, et al. The complete mitochondrial genome of Eriocheir japonica sinensis (Decapoda: Varunidae) and its phylogenetic analysis[J]. Biochemical Systematics and Ecology, 2015, 62: 241-248.
  6. Wei M, He L, Yang X, et al. The complete mitochondrial genome of Pseudobagrus albomarginatus Rendhal: sequencing and analysis[J]. Mitochondrial DNA, 2015, 26(4): 633-635.
  7. Zhang Q G, Liang Y H. A recurrent R936X mutation of CYLD gene in a Chinese family with multiple familial trichoepithelioma[J]. Indian Journal of Dermatology, Venereology and Leprology, 2015, 81: 192.
  8. Li G, Xu J, Wang H, et al. Identification and analysis of the complete mitochondrial genome sequence of Eriocheir sinensis[J]. Mitochondrial DNA Part A, 2016, 27(6): 3923-3924.
  9. Feng T, Li S, Wang S, et al. Cross priming amplification with nucleic acid test strip analysis of mutton in meat mixtures[J]. Food chemistry, 2018, 245: 641-645.
  10. Tang L, Wang J, Zhu J, et al. Down‐regulated SHARPIN may accelerate the development of atopic dermatitis through activating interleukin‐33/ST 2 signalling[J]. Experimental Dermatology, 2018, 27(12): 1328-1335.
  11. Lu S, Xu F, Hu W, et al. SCD1 methylation in subcutaneous adipose tissue associated with menopausal age[J]. Climacteric, 2019, 22(4): 395-402.
  12. Huang Z, Zhang Q, Wang Y, et al. Inhibition of caspase-3-mediated GSDME-derived pyroptosis aids in noncancerous tissue protection of squamous cell carcinoma patients during cisplatin-based chemotherapy[J]. American Journal of Cancer Research, 2020, 10(12): 4287.
  13. Liu H, Hua Z Q, Jin Z B. Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids[J]. STAR protocols, 2021, 2(2): 100444.
  14. Wang P, Christiana O, Yu L, et al. The complete mitochondrial genome of the common strain black carp (Cyprinus carpio var. baisenensis)[J]. Mitochondrial DNA Part B, 2021, 6(2): 542-545.
  15. Ke-**n Y, **ang C, Qing-Qing H, et al. Development of a tetra-primer ARMS–PCR for identification of sika and red deer and their hybrids[J]. Analytical Sciences, 2023, 39(12): 1947-1956.
  16. Shi Q, Fan X, Yang M, et al. Paternal preconceptional supplementation of n‐3 polyunsaturated fatty acids alleviates offspring nonalcoholic fatty liver disease in high‐fat diet‐induced obese mice[J]. Food Frontiers, 2024, 5(2): 535-557.
  17. H Ye, H Xu, J Xu, J Liang, X Wang, T Huang – Microchemical Journal, 2024 – Elsevier… The animal tissue DNA extraction kit was purchased from Simgen Biological Co., Ltd (Hangzhou, China). The RPA thermostatic rapid amplification kit was purchased from Amp Future …
  18. Wang Z, Zhou J, Lin L, et al. Discovery of a far-red carbazole-benzoindolium fluorescent ligand that selectively targets mitochondrial DNA and suppresses breast cancer growth[J]. European Journal of Medicinal Chemistry, 2024, 264: 116046.
  19. YuanN, Shen L, Peng Q, et al. SRSF1 Is Required for Mitochondrial Homeostasis and Thermogenic Function in Brown Adipocytes Through its Control of Ndufs3 Splicing[J]. Advanced Science, 2024: 2306871.
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