Viral Nucleic Acid Extraction Kit
Suitable for extract viral DNA or viral RNA from various bodily fluid samples.
Viral Nucleic Acid Extraction Kit
- Preparate viral nucleic acid within 20-40 min.
- Detection sensitivity of viral DNA is increased 10-50 timesthan the boiling extraction
- Detection sensitivity of viral RNA is increased 5-10 timesthan the traditional Trizol method.
- Carrier RNA increases the detection sensitivity (stable detection of virus at a concentration of 100 copies/ml in body fluids).
- No need of phenol chloroform and isopropanol.
- Nucleic acids are free of impurities and inhibitors and can be amplified using up to 1/2 the PCR reaction volume as
Price List
| Product No | Quantity | Price |
| 4002050 | 50 preps | contact us |
| 4002250 | 250 preps | contact us |
Description
The body fluid sample is digested by proteinase K to release viral nucleic acid, and then supplemented by column purification technology to adsorb viral nucleic acid on the spin column. After washing the spin column with a wash buffer, the impurities and PCR inhibitors remaining on the spin column can be completely removed. Viral nucleic acids on the spin column can be eluted directly with Buffer TE and can be used directly in various molecular biology experiments.
Specifications
- Samples: body fluids (plasma, serum, urine, CSF, and cell culture supernatant).
- Sample amount: 200 μl body fluid sample.
Experimental date
Figure 1: Amplification plot of HBV DNA
Curve 1: Nucleic acids extracted in serum by boiling method (5 μl DNA added in a 50 μl amplification system).
Curve 2: Nucleic acids extracted from the same serum (200 μl) with Simgen Virus Nucleic Acid Extraction Kit (5 μl DNA added in a 50 μl amplification system).
Curve 3: Nucleic acids extracted from the same serum (200 μl) with Simgen Virus Nucleic Acid Extraction Kit (25 μl DNA added in a 50 μl amplification system). No significant inhibition effect was observed, but the CT value was reduced.
Specifications
