Circulating Nucleic Acid Extraction Kit
Suitable for extracting cell-free nucleic acids from 1-5 ml plasma samples.
Circulating Nucleic Acid Extraction Kit
- Ultra-high sensitivity, stable detection of cell-free DNA in plasma at a concentration of 1~2 copies/ml (> 70 bp, 5 ml sample).
- Prepare plasma total nucleic acid within 1 h
- Carrier RNA ensures high recovery of trace nucleic acids.
- Concentration of plasma free nucleic acids is increased by more than 50-400 times than boiling method.
- Free of impurities and inhibitors and can be amplified using up to 1/2 the volume of the reaction template.
Price List
| Product No | Quantity | Price |
| 3113050 | 50 preps | contact us |
Description
Specially designed extension tubes ensure efficient recovery trace levels of nucleic acids from large volumes (1-5 ml) of plasma. Two specially designed wash solutions completely remove impurities and PCR inhibitors. The purified nucleic acids can be directly used in various molecular biology experiments.
Specifications
Application: Early diagnosis, prognosis assessment, recurrence detection of tumors, prenatal diagnosis of fetuses, Immune disease analysis, etc.
Samples: body fluids (including plasma, serum).
Sample volume: 1-5 ml.
Instruments required: negative pressure device, microcentrifuge.
Experimental data
Figure 1: Amplification plot of the SRY gene (male characteristic gene amplification product, about 70 bp).
DNA extracted after dilution of human plasma to 10 copies/ml used as a template.
1: 5 μl of nucleic acid extracted from the Simgen Circulating Nucleic Acid Extraction Kit was used as a template for the detection of the SRY gene.
2: 20 μl of nucleic acid extracted from the Simgen Circulating Nucleic Acid Extraction Kit was used as a template for the detection of the SRY gene.
3: 5 μl of nucleic acid extracted by the boiling method as a template for the detection of the SRY gene was shown negative in both tubes.
Specifications
Resources
Thesis
- Zhang S, Chen Z, Huang C, et al. Ultrasensitive and quantitative detection of EGFR mutations in plasma samples from patients with non-small-cell lung cancer using a dual PNA clamping-mediated LNA-PNA PCR clamp[J]. Analyst, 2019, 144(5): 1718-1724.
- ChenZ, Zhang S, Li C, et al. Comprehensive evaluation of the factors affecting plasma circulating cell-free DNA levels and their application in diagnosing nonsmall cell lung cancer[J]. Genetic Testing and Molecular Biomarkers, 2019, 23(4): 270-276.
- 张占会, 赵新景, 宋元宗, 等. 人羊水细胞中 SLC25A13 基因转录产物的克隆和序列分析[J]. 中国当代儿科杂志, 2012, 14(3): 221-225.
- Li J, Ding J, Xu F. Combination Analyses Next Generation Sequencing of Lung Adenocarcinoma ctDNA and CTCs Based on Multi-Site Immunomagnetic Beads[J]. Science of Advanced Materials, 2023, 15(4): 484-493.