One Step DNA Cloning Kit

A simple, fast, and efficient directional cloning kit.

One Step DNA Cloning Kit

The One Step DNA Cloning Kit allows for the assembly of one or multiple DNA fragments in a single reaction, with single-fragment insertion completed in as little as 5 minutes and a positive rate exceeding 95%.This Mix uses HiFi Taq DNA Ligase, which has higher fidelity compared to wild-type Taq DNA Ligase, significantly improving the success rate of seamless cloning. Additionally, the Mix includes a transformation enhancer, greatly increasing the number of transformants. While ensuring high fidelity and transformation efficiency the One Step DNA Cloning Kit has optimized its components to enhance stability, making it more stable under high temperature or oxidic conditions.

Price List

Cat. No. specification Price
E001020 20 Preps contact us
E001050 50 Preps contact us

Introduction

One Step DNA Cloning Kit is a kind of seamless assembly method digest, ligate or end repair are not required. This method has been used to assemble different sizes of DNA fragments with varied overlaps (15~30 bp). And it offers an easy, fast and efficient DNA cloning technique.

The One Step DNA Cloning Kit allows for the assembly of one or multiple DNA fragments in a single reaction, with single-fragment insertion completed in as little as 5 minutes and a positive rate exceeding 95%.This Mix uses HiFi Taq DNA Ligase, which has higher fidelity compared to wild-type Taq DNA Ligase, significantly improving the success rate of seamless cloning. Additionally, the Mix includes a transformation enhancer, greatly increasing the number of transformants. While ensuring high fidelity and transformation efficiency the One Step DNA Cloning Kit has optimized its components to enhance stability, making it more stable under high temperature or oxidic conditions.

Storage

It can be transported at Low temperature and stored at -20℃ for more than 2 years.

Features

Compatible: recombination of single or multiple DNA fragments can be achieved at a time, with some tolerance to impurities contained in unpurified PCR products.

Efficient: single-fragment reassembly takes as little as 5 min.

Reliable: the Gibson Cloning principle used has been validated in multiple papers.

Composition(Cat.No.7007005)

-20℃ storage

2×One Step DNA Cloning Mix 100 μl 250 μl
pUC19 Control Plasmid, Linearized (Amp+, 40 ng/μl) 5 μl 5 μl
500 bp Control Insert (20 ng/μl) 5 μl 5 μl

Resources

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