Circulating Nucleic Acid Extraction Kit
Suitable for extracting cell-free nucleic acids from 1-5 ml plasma samples.
Circulating Nucleic Acid Extraction Kit
- Ultra-high sensitivity, stable detection of cell-free DNA in plasma at a concentration of 1~2 copies/ml (> 70 bp, 5 ml sample).
- Prepare plasma total nucleic acid within 1 h
- Carrier RNA ensures high recovery of trace nucleic acids.
- Concentration of plasma free nucleic acids is increased by more than 50-400 times than boiling method.
- Free of impurities and inhibitors and can be amplified using up to 1/2 the volume of the reaction template.
Price List
| Product No | Quantity | Price |
| 3113050 | 50 preps | contact us |
Description
Specially designed extension tubes ensure efficient recovery trace levels of nucleic acids from large volumes (1-5 ml) of plasma. Two specially designed wash solutions completely remove impurities and PCR inhibitors. The purified nucleic acids can be directly used in various molecular biology experiments.
Specifications
Application: Early diagnosis, prognosis assessment, recurrence detection of tumors, prenatal diagnosis of fetuses, Immune disease analysis, etc.
Samples: body fluids (including plasma, serum).
Sample volume: 1-5 ml.
Instruments required: negative pressure device, microcentrifuge.
Experimental data
Figure 1: Amplification plot of the SRY gene (male characteristic gene amplification product, about 70 bp).
DNA extracted after dilution of human plasma to 10 copies/ml used as a template.
1: 5 μl of nucleic acid extracted from the Simgen Circulating Nucleic Acid Extraction Kit was used as a template for the detection of the SRY gene.
2: 20 μl of nucleic acid extracted from the Simgen Circulating Nucleic Acid Extraction Kit was used as a template for the detection of the SRY gene.
3: 5 μl of nucleic acid extracted by the boiling method as a template for the detection of the SRY gene was shown negative in both tubes.
Specifications
