Supercoiled DNA Ladder

Supercoiled DNA Ladder consists of 8 supercoiled plasmid DNA ranging from 2kb to 15kb. Supercoiled DNA Ladder contains 1×Loading Buffer, users can apply 5 – 10 μl in agarose gel electrophoresis directly.

Supercoiled DNA Ladder

Two optimized Loading Buffer dyes are added that do not obscure the brightness of the fluorescence of the DNA fragments.
Each finely adjusted fragment is clear enough.

Price List

Product No Quantity Price
MD1014 250 µl contact us
MD1114 1.25ml contact us

INTRODUCTION

Supercoiled DNA Ladder consists of 8 supercoiled plasmid DNA ranging from 2kb to 15kb. Supercoiled DNA Ladder contains 1×Loading Buffer, users can apply 5 – 10 μl in agarose gel electrophoresis directly.

The red and yellow tracking dye in Supercoiled DNA Ladder will not weakened the DNA bands under UV light, better than bromophenol blue and xylene cyanol FF.

PRECAUTION

  1. Clear bands can be obtained by applying 5 μl DNA Ladder when the lane width is less than 5 mm. If the lane is wider, loading volume of DNA Ladder should be increased appropriately.
  2. For DNA electrophoresis, agarose purity is of great significance to DNA band definition. Therefore, agarose with good quality should be used and gel concentration of 0.7%~1% is recommended.
  3. During agarose electrophoresis, the concentration of agarose is closely associated with the separation of DNA  High agarose concentration is ideal for the separation of the short DNA fragments. While low agarose concentration is ideal to separate the long DNA fragments.

 

 

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