Introduction

Cultured cells are lysed with lysis buffer and released RNA, then bind to the spin column without the addition of absolute ethanol, while the solubilized proteins and inhibitors are filtered out. After washing with the Buffer WBR, the RNA is eluted with RNase-free Water and can be used for various molecular biology experiments such as RT-PCR, Northern blot, Dot blot, mRNA isolation, etc.

Experimental data

Figure 1: Electrophoresis plot of RNA extracted from cultured cells with Simgen Rapid Cultured Cells Total RNA Extraction Kit.

1, 2: CHO-K cells.

3, 4: A594 cells.

Specifications