Pfu DNA Polymerase
Suitable for high-fidelity amplification of DNA, such as gene expression cloning, site-directed mutagenesis, analysis of intracellular point mutations (SNPs), and end-complementing.
Pfu DNA Polymerase
Pfu DNA Polymerase was isolated and purified from Escherichia coli cloned with the Pyrococcus furiosis DNA polymerase gene, Pfu DNA Polymerase has 5′→3′ DNA polymerase activity and 3′→5′ exonuclease activity to correct base mismatches generated during DNA amplification. The Pfu DNA Polymerase has the lowest error rate of all high-temperature DNA polymerases that have been discovered to date. The PCR product is blunt-end, which can be treated with A-Tailing and then ligated with a T vector or blunt-end cloning vector.
Price list
| Cat. No. | Specification (5 U/μl) | Price |
| 8007050 | 50 μL | contact us |
| 8007100 | 100 μL | contact us |
Introduction
Pfu DNA Polymerase was isolated and purified from Escherichia coli cloned with the Pyrococcus furiosis DNA polymerase gene, Pfu DNA Polymerase has 5′→3′ DNA polymerase activity and 3′→5′ exonuclease activity to correct base mismatches generated during DNA amplification. The Pfu DNA Polymerase has the lowest error rate of all high-temperature DNA polymerases that have been discovered to date. The PCR product is blunt-end, which can be treated with A-Tailing and then ligated with a T vector or blunt-end cloning vector.
Storage
It can be transported at low temperature and stored at -20℃ for more than 2 years.
Composition(Cat.No.8007050)
| Pfu DNA Polymerase | 50 μL |
| 10 × Pfu Buffer | 0.5 ml |
| ddH2O | 1 ml |
