Price List

Introduction

The active ingredient of bacterial cryopreservation solution is glycerol, which can be used for freezing and preserving various common bacteria. The bacterial strain can be stored for 1 year at -20ºC and 5-10 years at -80ºC.

Protocol

1. Use liquid medium such as LB to grow the bacteria to the logarithmic stage.
2. In a sterile environment such as a super–clean bench, the Bacterial Cryopreservation Solution and bacterial solution (including media) are added to the same sterilized freeze-storage tube or centrifuge tube at a 1:1 volume ratio. (Usually adding 500 μl Bacterial Cryopreservation Solution and 500 μl bacterial solution is sufficient for use.)

* Add the Bacterial Cryopreservation Solution first, then close the lid of the Bacterial Cryopreservation Solution, and then add the bacterial liquid, so as not to accidentally contaminate the Bacterial Cryopreservation Solution.

* You can pack more Bacterial Cryopreservation Solution into the sterilized freeze-up tube or centrifuge tube in advance and keep it for later use.

3. The bacteria can be stored at -20℃ or -80℃ after being thoroughly mixed upside down.

Precautions

• Pay attention to aseptic operation to avoid contamination by miscellaneous bacteria.
• The cryopreservation solution must be well mixed with bacteria, otherwise it will not be able to rise at low temperatures .to the role of protecting bacteria.