2×SYBR Green PCR Mix
Suitable for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription
2×SYBR Green PCR Mix
2× SYBR Green PCR Mix is a two-fold concentration master mix designed for use with fluorescence dye-based (SYBR Green I) real-time PCR. The product uses DNA polymerase of hot-start method of anti-Taq antibody, combined with PCR enhancer and protein stabilizer in the reaction system, which not only effectively avoids various non-specific amplification, but also greatly improves the amplification efficiency of PCR. The product is separately equipped with ROX dye, which is suitable for use in mainstream fluorescence PCR instruments such as Applied Biosystems, Bio-Rad, Eppendorf, Roche, etc. This product is suitable for fast Real Time PCR amplification reactions, and can obtain a good standard curve in a wide range of quantification regions, and accurately quantify and detect target genes, with good reproducibility and high confidence.
Price List
| Cat. No. | specification | Price |
| 7106100 | 1 ml | contact us |
| 7106500 | 5 ml | contact us |
Introduction
2× SYBR Green PCR Mix is a two-fold concentration master mix designed for use with fluorescence dye-based (SYBR Green I) real-time PCR. The product uses DNA polymerase of hot-start method of anti-Taq antibody, combined with PCR enhancer and protein stabilizer in the reaction system, which not only effectively avoids various non-specific amplification, but also greatly improves the amplification efficiency of PCR. The product is separately equipped with ROX dye, which is suitable for use in mainstream fluorescence PCR instruments such as Applied Biosystems, Bio-Rad, Eppendorf, Roche, etc. This product is suitable for fast Real Time PCR amplification reactions, and can obtain a good standard curve in a wide range of quantification regions, and accurately quantify and detect target genes, with good reproducibility and high confidence.
Storage
It can be transported at low temperature and stored at -20℃ for more than two years.
Experimental Data
Configure the PCR system according to the following components: Standard curve
| Component | volume |
| 2×SYBR Green PCR Mix | 25 μL |
| Forward primer (10 μM) | 1 μl |
| Reverse primer (10 μM) | 1 μl |
| ROX | 1 μl |
| Template DNA | 5 μl |
| ddH2O | Add to 50 μl |
Experimental Data
Configure the PCR system according to the following components: Standard curve
Figure 1: Standard Curve
V=-3.4054X+28.155
R2=0.9979
Figure 2: Amplification curve
Figure 3: Melting curve
Figure 1: Standard Curve
V=-3.4054X+28.155
R2=0.9979
Figure 2: Amplification curve
Figure 3: Melting curve
