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cDNA first-strand synthesis kit

Suitable for the synthesis of first-strand cDNA, cDNA library construction, SAGE (Serial Analysis of Gene Expression)

cDNA first-strand synthesis kit

cDNA first-strand synthesis kit

One tube operation, 21 min to obtain high-quality cDNA without genomic DNA contamination. This kit contains a new and efficient gDNase, which can efficiently remove genomic DNA in 3 min, so that genomic DNA can be efficiently removed before transcription. Efficient reverse transcriptase, which can synthesize first-strand cDNA for fluorescence quantification experiments in just 15 min, with a reversal efficiency of up to 90%.

Price List

 

Product No Quantity Price
7306025 25 preps contact us
7306100 100 preps contact us

Introduction

One tube operation, 21 min to obtain high-quality cDNA without genomic DNA contamination. This kit contains a new and efficient gDNase, which can efficiently remove genomic DNA in 3 min, so that genomic DNA can be efficiently removed before transcription. Efficient reverse transcriptase, which can synthesize first-strand cDNA for fluorescence quantification experiments in just 15 min, with a reversal efficiency of up to 90%.

Storage

This kit can be transported at low temperature, stored at -20℃ for more than 2 years.

Experimental Data

Figure 1: The image on the right shows the same initial amount of rat RNA of 1 μg each, reverse transcribed with cDNA kits from different companies, diluted 10-fold, and 5 μl used as PCR template for fluorescent PCR.

PCR primer: Rat β-actin Internal control primers

Forward：CCCATCTATGAGGGTTACGC

Reverse：TTTAATGTCACGCACGATTTC

Resources

Instructions
Instructions

Thesis

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