2×Probe qPCR Mix

Suitable for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

2×Probe qPCR Mix

2×Probe qPCR Mix is a two-fold concentration of master mix designed for probe-based real-time PCR. The product uses a hot-start DNA polymerase with anti-Taq antibody, which can be combined with the most suitable Buffer for Real Time PCR, which can greatly improve the amplification efficiency of PCR and perform high-sensitivity Real Time PCR amplification reactions. The product is separately equipped with ROX dye, which is suitable for use in mainstream fluorescence PCR instruments such as Applied Biosystems, Bio-Rad, Eppendorf, Roche, etc. This product is suitable for fast Real Time PCR amplification reactions and can obtain a good standard curve in a wide range of quantification regions, and accurately quantify and detect target genes, with good reproducibility and high confidence.

Price List 

Cat. No. specification Price
7206100 1 ml contact us
7206500 5 ml contact us

Introduction

2×Probe qPCR Mix is a two-fold concentration of master mix designed for probe-based real-time PCR. The product uses a hot-start DNA polymerase with anti-Taq antibody, which can be combined with the most suitable Buffer for Real Time PCR, which can greatly improve the amplification efficiency of PCR and perform high-sensitivity Real Time PCR amplification reactions. The product is separately equipped with ROX dye, which is suitable for use in mainstream fluorescence PCR instruments such as Applied Biosystems, Bio-Rad, Eppendorf, Roche, etc. This product is suitable for fast Real Time PCR amplification reactions and can obtain a good standard curve in a wide range of quantification regions, and accurately quantify and detect target genes, with good reproducibility and high confidence.

Storage

It can be transported at low temperature and stored at -20℃ for more than 2 years.

Experimental Data

Figure 1: Amplification plot of 10-fold, 100-fold, 1000-fold, and 10,000-fold dilution of porcine DNA with porcine-specific primers and probes and Simgen 2×Probe qPCR Mix.

Composition(Cat.No.7206100)

Store at -20°C

2×Probe qPCR Mix 1 ml
50×ROX Reference Dye 40 μL
ddH2O 1 ml

Resources

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