Buffer EX
It does not need to be operated in a chemical fume hood.No need for a cumbersome purchase process.
Introduction
Buffer EX is a mixed solution prepared from various non-toxic or low-toxicity organic solvents, which is low toxicity (close to the toxicity of isopropanol) and not easy to volatile. For RNA extraction, which can perfectly replace chloroform to extract phenol and fats from biological samples dissolved by Trizol reagent. Due to the low toxicity and non-volatile nature of Buffer EX, users do not need to operate in a fume hood if they use Trizol Reagent for RNA extraction.
Melting Point: -41.8°C
Boiling point: 178.3 °C
Solubility(water): 1.16 g/100 ml (20℃)
Density: 1.12~1.19 g/ml
Appearance: Colorless liquid with a special odor.
Flash Point: 84.4°C
LD50: (rat, oral) 4.293 g/kg
Experimental Data
Table 1: Measured of RNA extracted from mouse liver with Simgen Trizol.
Figure 1: electrophoresis plot of the above RNA in table 1.
M:Simgen DL2000 Ladder
1, 2: RNA extracted with chloroform.
1, 2: RNA extracted with Buffer EX.