Blood Total RNA Kit
Suitable for extracting total RNA from 500 μl fresh or -80℃ frozen whole blood and bone marrow.
Blood Total RNA Kit
- Preparetotal RNA from whole blood in 20-40 min.
- No need to remove red blood cells.
- Whole blood samples can be lysed with Buffer L9and stored at -20℃ for one week before use.
- RNA is free of impurities and inhibitors and can be used up to 1/2 of the volume of the reaction system.
Price List
| Product No | Quantity | Price |
| 5201050 | 50 preps | contact us |
Description
Whole blood or bone marrow samples are lysed in a strong lysis buffer and precipitated to remove proteins. Add ethanol to the supernatant contained RNA, then add mixture to the spin column, the RNA was bound to the spin column. Residual proteins and inhibitors are filtered out, washed by Buffer WA and Buffer WBR, and eluted with RNase-Free water; the RNA can be used for various molecular biology experiments.
Specifications
Sample: 500 μl fresh or -80℃ frozen whole blood, bone marrow.
Elution volume: 50 μl.
Recovery: Up to 90%.
Applications: RT-PCR, Northern blot, Dot blot, mRNA isolation, blood RNA virus test, blood leukocyte fusion gene test.
Experimental data
Figure 1: RNA extracted from 500 μl whole blood (1-4 are the same sample) using the Simgen Blood Total RNA Extraction Kit.
Figure 2: Electrophoresis plot of RNA extracted by different method.
1, 2: RNA extracted from 500 μl leukocytes in whole blood (red blood cells removed by Red Blood Cell Lysis Buffer) using Trizol method.
3-6: RNA extracted from 500 μl the same whole blood sample using the Simgen Blood Total RNA Extraction Kit.
Publications
