info@simgen.cn

Ultra Pure Total RNA Extraction Kit

Suitable for extracting total RNA from various different sample types.

Ultra Pure Total RNA Extraction Kit

Ultra Pure Total RNA Extraction Kit

  • Prepare total RNA in 20-40 min.
  • The perfect combination of acidic phenol extraction and column purification.
  • No need of chloroform, and Buffer EX is configured as a perfect alternative to chloroform.
  • Samples: animal tissues, cells, plant tissues, blood, etc.
  • Applications: RT-PCR, Northern Blot, chip analysis, etc.

Price List

Product No Quantity Price
5003050 50 preps contact us

Description

This product organically combines the guanidine thiocyanate/acid phenol extraction RNA method with column purification technology and is equipped with chloroform replacement reagent Buffer EX to remove genomic DNA by acid phenol extraction. The entire RNA extraction process is equivalent to two purifications, extraction purification and column purification, and finally the high-purity RNA eluted with RNase-free water can be immediately used in various molecular biology experiments.

Examples of experiments

Figure 1: RNA extracted from 1 ml freshly cultured Bacillus subtilis (1-4 are the same sample) using the Simgen Ultra Pure Total RNA Extraction Kit.

Figure 2: In step 2 of the Simgen Ultra Pure Total RNA Extraction Kit, the RNA to be extracted is in the upper aqueous phase.

figure 3: amplification plot of RNA extracted from 50 mg rat muscle tissue (1-4 are the same sample) with Simgen Ultra Pure Total RNA Extraction Kit, treated with Simgen cDNA First Strand Synthesis Kit, and amplified by PCR with Rat-β-actin primers.

Specifications

Resources

Instructions
Instructions

Thesis

  1. Zhang M, Li G, Sun X, et al. MET amplification, expression, and exon 14 mutations in colorectal adenocarcinoma[J]. Human pathology, 2018, 77: 108-115.
  2. 龚仲幸, 何勇, 杨静, 等. 外源硫酸铝调节八仙花花青苷组成和含量变化的分子生物学机制[J]. 植物营养与肥料学报, 2017, 23(3): 821-826.
  3. Rong J, Cheng T, Liu X, et al. Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens[J]. Vaccine, 2005, 23(40): 4844-4851.
  4. Li G, Liu L, Xu B, et al. Displaying epitope B and epitope 7 of porcine reproductive and respiratory syndrome virus on virus like particles of porcine circovirus type 2 provides partial protection to pigs[J]. Journal of Veterinary Medical Science, 2021, 83(8): 1263-1272.
  5. Diao X, Han Y, Liu C. The fungicidal activity of tebuconazole enantiomers against Fusarium graminearum and its selective effect on DON production under different conditions[J]. Journal of agricultural and food chemistry, 2018, 66(14): 3637-3643.
  6. AyazA, Huang H, Zheng M, et al. Molecular cloning and functional analysis of GmLACS2-3 reveals its involvement in cutin and suberin biosynthesis along with abiotic stress tolerance[J]. International Journal of Molecular Sciences, 2021, 22(17): 9175.
  7. Wang X, Zhu H, He Y, et al. A novel homozygous mutation in the PADI6 gene causes early embryo arrest[J]. Reproductive Health, 2022, 19(1): 190.
  8. Li J, Zhang T, Liu K, et al. Protective effects and mechanisms of Yi Qi Huo Xue Fang in cerebral ischemic stroke based on network pharmacology and experimental verification[J]. Journal of Ethnopharmacology, 2023, 314: 116611.
  9. Mu Z, Shen S, Tang L, et al. Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions[J]. Heliyon, 2023, 9(1): e12958.
  10. Yang Y, Cheng J, Han H, et al. Genome-wide identification of the HKT transcription factor family and their response to salt stress in foxtail millet (Setaria italica)[J]. Plant Growth Regulation, 2023, 99(1): 113-123.
  11. Li H, Qin Y, Huang Y, et al. SPAG5, the upstream protein of Wnt and the target of curcumin, inhibits hepatocellular carcinoma[J]. Oncology Reports, 2023, 50(3): 1-10.
  12. Liu Y, Guo W, Hong S L. Aerobic exercise mitigates hippocampal neuronal apoptosis by regulating DAPK1/CDKN2A/REDD1/FoXO1/FasL signaling pathway in D‐galactose‐induced aging mice[J]. The FASEB Journal, 2023, 37(10): e23205.
  13. Lu Q, ** L, Wang P, et al. Effects of Interaction of Protein Hydrolysate and ArbuscularMycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress[J]. Journal of Fungi, 2023, 9(10): 983.
  14. Zhou C, Li J, Zheng C, et al. Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion——From the perspective of glial-vascular unit[J]. Journal of Ethnopharmacology, 2024, 318: 116990.
  15. Wang K, Mei Z, Zheng M, et al. FTO-mediated autophagy inhibition promotes non-small cell lung cancer progression by reducing the stability of SESN2 mRNA[J]. Heliyon, 2024, 10(5).
  16. Liu Y, Meng X K, Shao W, et al. miR-34a/TAN1/CREB Axis Engages in Alleviating Oligodendrocyte Trophic Factor-Induced Myelin Repair Function and Astrocyte-Dependent Neuroinflammation in the Early Stages of Alzheimer’s Disease: The Anti-Neurodegenerative Effect of Treadmill Exercise[J]. Neurochemical Research, 2024, 49(4): 1105-1120.
  17. Liu Y, Meng X, Tang C, et al. Aerobic exercise modulates RIPK1-mediated MAP3K5/JNK and NF-κB pathways to suppress microglia activation and neuroinflammation in the hippocampus of D-gal-induced accelerated aging mice[J]. Physiology & Behavior, 2024, 286: 114676.
  18. Zeng F, Wang M, Li Z, Zhang Y. Overexpression of the circadian gene Bmal1 regulates the Nrf2/HO-1 oxidative stress pathway to alleviate inflammation and apoptosis in PC12 cells following cerebral ischemia-reperfusion injury[J]. Medicine. 2025 13;104(24):e42763.
  19. Zhou X, Ma D, He YX, ** J, Wang HL, Wang YF, Yang F, Liu JQ, Chen J, Li Z. Kangfuxin solution alleviates esophageal stenosis after endoscopic submucosal dissection: A natural ingredient strategy[J]. World Journal of Gastroenterology. 2025 7;31(1):98561.
Scroll to Top