RNAisol
For extracting RNA from various samples.
Price List
| Product No | Quantity | Price |
| 5301100 | 100 ml | contact us |
Introduction
RNAisol is a ready-to-use reagent for extracting total RNA from samples such as cells and tissues based on the guanidine isothiocyanate/phenol method, which preserves RNA integrity while lysing cells and dissolving cellular inclusions during sample lysis or homogenization. With the addition of chloroform or the chloroform replacement reagent Simgen Buffer EX (Cat. No. 9025-100), the solution is divided into aqueous, mesosphere, and organic phases, with RNA in the aqueous phase. The aqueous phase was taken out, and RNA could be recovered by precipitation with isopropanol. The middle layer is precipitated with ethanol to recover DNA. The organic phase precipitates the recoverable protein with isopropanol.
Specifications

Resources
Thesis
- Chen X M, Lu H M, Niu X T, et al. Enhancement of secondary metabolites from Bacillus Licheniformis XY-52 on immune response and expression of some immune-related genes in common carp, Cyprinus carpio[J]. Fish & shellfish immunology, 2015, 45(1): 124-131.
- 宗鑫, 胡汪洋, 汪以真. 猪髓样分化因子 MyD88 特异性 shRNA 干扰载体的构建筛选及干扰效果评价[J]. 中国生物工程杂志, 2015, 35(7): 1-7.
- Yao J Y, Xu Y, Yuan X M, et al. Proteomic analysis of differentially expressed proteins in the two developmental stages of Ichthyophthirius multifiliis[J]. Parasitology research, 2017, 116(2): 637-646.
- Chen XM, Guo G L, Sun L, et al. Modulatory role of L-carnitine against microcystin-LR-induced immunotoxicity and oxidative stress in common carp[J]. Fish physiology and biochemistry, 2017, 43: 1081-1093.
- Li M Y, Sun L, Niu X T, et al. Astaxanthin protects lipopolysaccharide-induced inflammatory response in Channa argus through inhibiting NF-κB and MAPKs signaling pathways[J]. Fish & shellfish immunology, 2019, 86: 280-286.
- Chen X, Zhang X, Zhao J, et al. Split iron supplementation is beneficial for newborn piglets[J]. Biomedicine & Pharmacotherapy, 2019, 120: 109479.
- Yin B, Ren H, Cai H, et al. Dynamics of cardiomyocyte and muscle stem cell proliferation in pig[J]. Experimental Cell Research, 2020, 388(2): 111854.
- Gao S T, Yu Y M, Wan L P, et al. LncRNA GAS5 induces chondrocyte apoptosis by down-regulating miR-137[J]. Eur Rev Med Pharmacol Sci, 2020, 24(21): 10984-10991.
- Wu J, Yin Y, Qin M, et al. The Effects of Vagus Nerve Stimulation Mediated Protection of Intestinal Epithelial Glycocalyx on Acute Lung Injury Associated With Traumatic Hemorrhagic Shock/Resuscitation[J]. 2020.J Wu, Y Yin, M Qin, K Li, F Liu, X Zhou, X Song, B Li – 2020 – researchsquare.com
- Huang P, Gu X J, Huang M Y, et al. Down-regulation of LINC00667 hinders renal tubular epithelial cell apoptosis and fibrosis through miR-34c[J]. Clinical and Translational Oncology, 2021, 23(3): 572-581.
- Liu B, Xu D, Ding X, et al. LncRNA SNHG1 improves chondrocyte apoptosis and inflammation microenvironment by down-regulating miR-195-5p[J]. Revista Argentina de Clínica Psicológica, 2020, 29(3): 1118.
- Song F, Yang Y, Jixiang Liu J. MicroRNA548ac induces apoptosis in laryngeal squamous cell carcinoma cells by targeting transmembrane protein 158[J]. Oncology Letters, 2020, 20(4): 1-1.
- Yu J,An Y, Zou M, et al. Mechanism of circular RNA RAD23B for regulating glycolysis and proliferation of ovarian cancer cells by targeting microRNA-519b-3p[J]. Journal of Biomaterials and Tissue Engineering, 2021, 11(6): 1078-1083.
- Wu J, Yin Y, Qin M, et al. Vagus nerve stimulation protects enterocyte glycocalyx after hemorrhagic shock via the cholinergic anti-inflammatory pathway[J]. Shock (Augusta, Ga.), 2021, 56(5): 832.
- Yu Z, Zhao Y Y, Jiang N, et al. Bioflocs attenuates lipopolysaccharide-induced inflammation, immunosuppression and oxidative stress in Channa argus[J]. Fish & Shellfish Immunology, 2021, 114: 218-228.
- A transcription factor DAF-5 functions in Haemonchus contortus developmentW Di, F Li, L He, C Wang, C Zhou… – Parasites & …, 2021 – parasitesandvectors.biomedcentral …
- Jiang Y, Peng Z, Man Q, et al. H3K27ac chromatin acetylation and gene expression analysis reveal sex-and situs-related differences in developing chicken gonads[J]. Biology of sex Differences, 2022, 13(1): 1-17.
- 黄颖,谢杰, 郑诗瑶, 等. 中国南方一罕见迟发性脊椎骨骺发育不良大家系的表型和基因型的相关性研究[J]. 中山大学学报 (医学版), 2022, 43(3): 392.2 – researchgate.net
- Jiang R, Zhang J, Liao Z, et al. Temperature-regulated type II grass carp reovirus establishes latent infection in Ctenopharyngodon idella brain[J]. Virologica Sinica, 2023.
- Song T, Hu Z, Zeng C, et al. FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas[J]. Heliyon, 2023, 9(6).
- Peng Z, Man Q, Meng L, et al. A PITX2-HTR1B pathway regulates the asymmetric development of female gonads in chicken[J]. PNAS Nexus, 2023: pgad202.
- Zhu W, Qiao M, Hu M, et al. Type II Grass Carp Reovirus Rapidly Invades Grass Carp (Ctenopharyngodon idella) via Nostril-Olfactory System-Brain Axis, Gill, and Skin on Head[J]. 2023.
- Yin B, Zhang K, Du X, et al. Developmental switch from morphological replication to compensatory growth for salamander lung regeneration[J]. Cell Proliferation, 2023, 56(3): e13369.
- Jiang R, Zhang J, Liao Z, et al. Temperature-regulated type II grass carp reovirus establishes latent infection in Ctenopharyngodon idella brain[J]. Virologica Sinica, 2023.
- Duan Y, ** C, Wu Y, et al. CREB1 alleviates the apoptosis and potentiates the osteogenic differentiation of zoledronic acid-treated human periodontal ligament stem cells via up-regulating VEGF[J]. Tissue and Cell, 2023, 85: 102223.
- Zhang K, Chen J, Liang L, et al. Lcn2 deficiency accelerates the infection of Escherichia coli O157: H7 by disrupting the intestinal barrier function[J]. Microbial Pathogenesis, 2023, 185: 106435.
- **a N, Zhang Y, Zhu W, et al. GCRV-II invades monocytes/macrophages and induces macrophage polarization and apoptosis in tissues to facilitate viral replication and dissemination[J]. Journal of Virology, 2024, 98(3): e01469-23.
- Hu T,Zeng C, Song Z, et al. HNRNPA2B1 and HNRNPR stabilize ASCL1 in an m6A-dependent manner to promote neuroblastoma progression[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2024, 1870(3): 167050
- Wang H, Cheng K, Li T, et al. A highly efficient Agrobacterium rhizogenes-mediated hairy root transformation method of idesia polycarpa and the generation of transgenic plants[J]. Plants, 2024, 13(13): 1791.
- Miao L, Tian C, Xiong Q, et al. Sex-Specific Appetite Regulation of Lipocalin-2 in High-Fat-Diet-Induced Obese Mice[J]. Neuroendocrinology, 2024, 114(5): 468-482.
