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Rapid Plasmid DNA Mini Kit

For extracting plasmid DNA up to 30 μg of molecular biology purity within 8 min.

Rapid Plasmid DNA Mini Kit

Rapid Plasmid DNA Mini Kit

  • Plasmid DNA can be prepared within 8 min.
  • No need of phenol and chloroformextraction.
  • The revolutionary innovation Buffer N8 provides a floating-free supernatant within 2 min.
  • Spin columns can be used without equilibration solution.
  • The special addition of neutralization indicators allows first-time users to obtain the best plasmid extraction results.

Price List

Product No Quantity Price
1005050 50 preps contact us
1005250 50 preps contact us

Introduction

Based on the column purification plasmid kit, the Buffer N8 solution is designed to extract up to 30 μg of high-purity plasmid DNA from 1-5 ml of bacterial culture within 8 min without changing the user’s plasmid extraction habits.

Specifications

  • Applications: Sequencing, restriction digestion, ligation and transformation, in vitro transcription/translation, transfection of strong cells
  • Plasmid DNA can be prepared within 8 min.
  • No need of phenoland chloroform extraction.
  • Starting volume: 1-5 ml bacterial culture.
  • Binding capacity: Up to 30 μg.
  • Instruments required: A high-speed centrifuge that with rotor for 2 ml tubes.

Experimental data

 

Figure 1: 2 min centrifugation of the fast plasmid kit

Figure 2: centrifugation for 10 min with a common plasmid kit

  • C: Electrophoresis effect of empty plasmidpUC-19.
  • 1: dimeric plasmid DNA. 2: nicked plasmid DNA.3: supercoiled plasmid DNA.
  • A: Single digestion effect of empty vector pUC-19.
  • B: Rendering of pUC-19 double digestion with insert

Specifications

Resources

Instructions
Instructions

Thesis

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Y Hu, M Huang, J Wen, J Gao, W Long, Y Shen… – Frontiers in …, 2023 – frontiersin.org

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