Storage

This product can be stored at -20℃ for up to 2 years.

Technical Support

R&D Department, Hangzhou Simgen Biotechnology Co., Ltd. E-mail: technical@simgen.cn, Tel: 400-0099-857.

Introduction

MinuteCut^TM enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All MinuteCut^TM enzymes show superior activity in the universal 10×MinuteCut^TM Buffer and 10×MinuteCut^TM Red Buffer and are able to digest DNA in 5~15 min. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. MinuteCut^TM enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. 10×MinuteCut^TM Red Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the 10×MinuteCut^TM Red Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Definition of Activity Unit

One unit of activity refers to the amount of enzyme required to completely digest 1 µg of pBR322 in a 50 μl reaction system at 37℃ for 1 hour.

Quality Control

Prolonged Incubation/Star Activity Assay

Under optimal reaction temperature, incubate 10 U Kas Ⅰ with 1 μg pBR322 for 16 hours. No contamination from other nucleases or nonspecific substrate degradation caused by star activity was detected. Longer incubation may result in star activity.

Ligation and Recutting

Under optimal reaction temperature, digest the substrate using 10 U Kas Ⅰ and recover the digested products. >95% of the DNA fragments can be ligated with T4 DNA Ligase at 22℃ .Of these ligated fragments, >95% can be recut with Kas Ⅰ as determined by agarose gel electrophoresis