MinuteCut Bsa Ⅰ Instructions
MinuteCutTM restriction enzyme has been genetically engineered and recombinant, and can quickly and accurately complete DNA digestion in 5~15 min.
MinuteCut Bsa Ⅰ Instructions
Suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA.
The dephosphorylated, ligation reagent is 100% active in MinuteCutTM digestion buffer.
It supports one-tube reaction to improve the experience of “digestion-modification-ligation”.
Price List
| Product No | Quantity | Price |
| 6025050 | 50 preps | contact us |
Storage
This product can be stored at -20℃ for up to 2 years.
Technical Support
R&D Department, Hangzhou Simgen Biotechnology Co., Ltd. E-mail: technical@simgen.cn, Tel: 400-0099-857.
Introduction
MinuteCutTM enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All MinuteCutTM enzymes show superior activity in the universal 10×MinuteCutTM Buffer and 10×MinuteCutTM Red Buffer and are able to digest DNA in 5~15 min. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. MinuteCutTM enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. 10×MinuteCutTM Red Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the 10×MinuteCutTM Red Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
Quality Control
Functional Test
A 20 μl reaction in 10×MinuteCutTM Buffer containing 1 μg of pPIC9K DNA and 1 μl of MinuteCutTM Bsa Ⅰ incubated for 15 min at 37℃ results in complete digestion as determined by agarose gel electrophoresis.
Prolonged Incubation/Star Activity Assay
A 20 μl reaction in 10×MinuteCutTM Buffer containing 1 μg of pPIC9K DNA and 1 μl of MinuteCutTM Bsa Ⅰ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.
Ligation and Recutting
After 10-fold over-digestion with MinuteCutTM Bsa Ⅰ at 37℃, >95% of the DNA fragments can be ligated with T4 DNA Ligase at 22℃. Of these ligated fragments, >95% can be recut with MinuteCutTM Bsa Ⅰ as determined by agarose gel electrophoresis.
Non-specific Endonuclease Activity
A 20 μl reaction in 10×MinuteCutTM Buffer containing 1 μg of supercoiled plasmid without the restriction site and 1 μl of MinuteCutTM Bsa Ⅰ incubated for 4 hours at 37℃ results in <10% conversion to the nicked or linearized form as determined by agarose gel electrophoresis.
