Hypersensitive Reverse Transcriptase
Suitable for first-strand cDNA synthesis, cDNA library construction, one-step RT-PCR, primer extension, 3′ and 5′ RACE, etc.
Hypersensitive Reverse Transcriptase
Third-generation M-MLV reverse transcriptase obtained by genetic modification and recombinant technology. The enzyme removed RNase H activity relative to wild-type M-MLV reverse transcriptase, and greatly improved reverse transcription speed and thermal stability (maximum tolerance temperature of 60°C), enhancing tolerance to the complex secondary structure of RNA. High-sensitivity reverse transcriptase has strong anti-interference and very high reverse transcription efficiency for low-copy RNA templates, and is the preferred reverse transcriptase for RT-PCR nucleic acid detection kits.
Price List
| Product No | Quantity | Price |
| 8106050 | 50 ul | contact us |
| 8106200 | 200 ul | contact us |
Introduction
Third-generation M-MLV reverse transcriptase obtained by genetic modification and recombinant technology. The enzyme removed RNase H activity relative to wild-type M-MLV reverse transcriptase, and greatly improved reverse transcription speed and thermal stability (maximum tolerance temperature of 60°C), enhancing tolerance to the complex secondary structure of RNA. High-sensitivity reverse transcriptase has strong anti-interference and very high reverse transcription efficiency for low-copy RNA templates, and is the preferred reverse transcriptase for RT-PCR nucleic acid detection kits.
Transportation & Storage
It can be transported at low temperature, stored at -20℃ for more than 2 years.
Features
- The half-life of 55℃is up to 60 min, and the maximum tolerance is 60°C
- Up to 10 kb of cDNA can be synthesized in 5 min, making it suitable for rapid reverse transcription reactions
- Very high tolerance to impurities, and a highly sensitive reverse transcriptase of choice for molecular diagnostics
Experimental Data
Figure 1: amplification plot of high-sensitivity reverse transcriptase applied to a one-step RT-qPCR reaction of COVID-19 pseudovirus RNA.
Composition（Cat.No.8106050）
| Highly sensitive reverse transcriptase | 50 μL |
| 5×RT Buffer | 0.5 ml |
| RNase-free Water | 1 ml |
