Hot Start Taq DNA polymerase
For genome amplification with high sensitivity and strong background (such as the detection of a specific gene site or pathogens), DNA sequence determination, Multiplex PCR, TA cloning, etc.
Hot Start Taq DNA polymerase
Hot-start Taq DNA polymerase is a solution that mixes a monoclonal antibody against Taq DNA polymerase with Taq DNA polymerase in 1:1 unit. The Taq antibody has a very high affinity for Taq DNA polymerase and can block the activity of Taq DNA polymerase before denaturation at high temperatures, so it can effectively inhibit primer-dimers and non-specific amplification, greatly improving the accuracy of PCR reactions. Hot-start Taq DNA polymerases can be used under conventional PCR reaction conditions without special high-temperature treatment, and are suitable for a variety of hot-start PCR and qPCR reactions based on Taq DNA polymerases. The 3′ end of the PCR product is Adenine base, which can be cloned directly with TA vector.
Price list
| Cat. No. | specification | Price |
| 8005100 | 100 μL | contact us |
| 8005500 | 500 μL | contact us |
Introduction
Hot-start Taq DNA polymerase is a solution that mixes a monoclonal antibody against Taq DNA polymerase with Taq DNA polymerase in 1:1 unit. The Taq antibody has a very high affinity for Taq DNA polymerase and can block the activity of Taq DNA polymerase before denaturation at high temperatures, so it can effectively inhibit primer-dimers and non-specific amplification, greatly improving the accuracy of PCR reactions. Hot-start Taq DNA polymerases can be used under conventional PCR reaction conditions without special high-temperature treatment, and are suitable for a variety of hot-start PCR and qPCR reactions based on Taq DNA polymerases. The 3′ end of the PCR product is Adenine base, which can be cloned directly with TA vector.
Storage
It can be transported at low temperature and stored at -20℃ for more than 2 years.
Composition(Cat.No.8005100)
热启动Taq DNA polymerase(2.5 U/µl)
| Hot Start Taq DNA polymerase | 100 μL |
| 10× PCR Buffer (Mg2+ plus) | 0.5 ml |
| ddH2O | 1 ml |
