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DNA Gel Extraction Kit

For recovery of 70 bp-10 kb of DNA from normal/low melting point agarose gels prepared with TAE or TBE buffers

DNA Gel Extraction Kit

DNA Gel Extraction Kit

  • Recovery of DNA in 20-30 min.
  • Recycling efficiency up to 85%.
  • The spin column does not require equilibration solution treatment.
  • Binding capacity: up to 20 μg of DNA.
  • The pH indicator contained in the solution provides a visual indication of the optimal binding conditions for DNA to the silica membrane in the solution.
  • Specially designed spin columns to elute DNA with a 25 μl microelution volume.
  • Applications: DNA sequencing, microarray analysis, DNA ligation and transformation, restriction digestion, labeled microinjection, PCR, in vitro transcription.

Price List

Product No Quantity Price
2001050 50 preps contact us
2001250 250 preps contact us

Introduction

After the DNA in the gel is released, it can be directly added to the spin column, and the gel molecules and other impurities dissolved by centrifugation are filtered and removed, and the DNA adsorbed on the spin column can be directly eluted with TE solution.

Experimental data

Figure 1: electrophoresis plot of DNA recovered with Simgen DNA Gel Extraction Kit.

30 μl of 100 bp DNA Marker was added to 120 μl of melted 1.5% agarose gel to make gel blocks containing DNA fragments of different lengths, recovered by Simgen Gel DNA Extraction Kit, and then eluted with 30 μl Buffer TE (a, b, c, d), and compared with the starting DNA Marker (70%, 100%) to control the recovery efficiency of each fragment. 1.5% TAE agarose gel.

Specifications

Resources

Instructions
Instructions

Thesis

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