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Description

Whole blood or bone marrow samples are lysed in a strong lysis buffer and precipitated to remove proteins. Add ethanol to the supernatant contained RNA, then add mixture to the spin column, the RNA was bound to the spin column. Residual proteins and inhibitors are filtered out, washed by Buffer WA and Buffer WBR, and eluted with RNase-Free water; the RNA can be used for various molecular biology experiments.

Specifications

Sample: 500 μl fresh or -80℃ frozen whole blood, bone marrow.

Elution volume: 50 μl.

Recovery: Up to 90%.

Applications: RT-PCR, Northern blot, Dot blot, mRNA isolation, blood RNA virus test, blood leukocyte fusion gene test.

Experimental data

Figure 1: RNA extracted from 500 μl whole blood (1-4 are the same sample) using the Simgen Blood Total RNA Extraction Kit.

Figure 2: Electrophoresis plot of RNA extracted by different method.

1, 2: RNA extracted from 500 μl leukocytes in whole blood (red blood cells removed by Red Blood Cell Lysis Buffer) using Tr**ol method.

3-6: RNA extracted from 500 μl the same whole blood sample using the Simgen Blood Total RNA Extraction Kit.

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