All-in-one First Strand cDNA Synthesis Kit (gDNA Purge)
An upgraded version of the cDNA First-Strand Synthesis Kit, which provides easy and fast operation for gDNA removal and RNA reverse transcription in one tube
All-in-one First Strand cDNA Synthesis Kit (gDNA Purge)
All-in-one First Strand cDNA Synthesis Kit（gDNA Purge）is an upgraded version of the cDNA First-Strand Synthesis Kit, which can perform both genomic DNA digestion and RNA reverse transcription reactions in the same tube, which is easy to use, and can effectively reduce the risk of sample contamination and RNA degradation caused by complex loading processes. In addition, the kit also provides No RT Control Enzyme Mix, which can be used to configure a control group without reverse transcriptase to determine whether genomic DNA remains in the RNA template.
Price List
| Product No | Quantity | Price |
| 7316050 | 50 preps | contact us |
| 73160100 | 100 preps | contact us |
Introduction
All-in-one First Strand cDNA Synthesis Kit（gDNA Purge）is an upgraded version of the cDNA First-Strand Synthesis Kit, which can perform both genomic DNA digestion and RNA reverse transcription reactions in the same tube, which is easy to use, and can effectively reduce the risk of sample contamination and RNA degradation caused by complex loading processes. In addition, the kit also provides No RT Control Enzyme Mix, which can be used to configure a control group without reverse transcriptase to determine whether genomic DNA remains in the RNA template.
Storage
It can be stored at -20℃ for 1 year.
Features
- gDNA removal and RNA reverse transcription in one tube, easy and fast.
- The use of high-temperature resistant reverse transcriptase greatly improves thermal stability and cDNA synthesis efficiency.
- Contains high potency dsDNase, which can specifically remove genomic DNA contamination and will not digest primers, RNA and subsequent synthesis of cDNA.
- The added reverse transcriptase has no RNase H activity, which can avoid RNA degradation in the RNA/DNA hybridization template strand during the first-strand cDNA synthesis process and ensure the quality of cDNA synthesis.
Experimental data
Figure 1: Amplification plot of the same initial amount of total rat RNA (1 μg and 10 ng), reverse transcribed with All-in-one First Strand cDNA Synthesis Kit (gDNA Purge) from different companies, and 2 μl as template for fluorescent PCR (50 μl system).
PCR Primer: Rat β-actin internal control.
