Rapid Total RNA Extraction Kit
High purity total RNA can be rapidly extracted from 106-107 animal cultured cells and 25-100 mg of animal tissue in 15 minutes.
Rapid Total RNA Extraction Kit
- Extremely simple operation steps, the extraction of tissue RNA can be completed within 10-15 minutes
- The lysis buffer RLF can efficiently dissolve biological samples, while genomic DNA, pigments, proteins and other impurities are centrifuged to form precipitates and.
- The specially designed Buffer WN efficiently removes salt residue, improving the OD260/OD230 ratio of RNA.
- The large-capacity RNA purification column is saturated with an adsorption efficiency of up to 100 μg or more
- Suitable for RNA extraction from 106-107 cultured animal cells, 25-100 mg animal tissue.
- Suitable for a variety of molecular biology experiments such as RT-PCR, Northern blot, Dot blot, mRNA separation, etc.
Introduction
This product uses the latest RNA column purification technology, which can quickly extract high-purity total RNA from 106-107 animal cultured cells or 25~50 mg animal tissues. The special Buffer RLF can efficiently lyse biological samples, and impurities such as genomic DNA, pigments, and proteins are centrifuged to form a precipitate. The adsorbed RNA on the Spin Column is washed with Buffer WN and Buffer WBR, then dissolved in RNase-free water, which can be used for various molecular biology experiments such as RT-PCR, Northern blot, Dot blot, mRNA extraction, etc.
Experimental data
L1、L2：RNA extracted from 25 mg crucian carp liver；M1，M2：RNA extracted from 50 mg crucian carp muscle. All the RNA were eluted with 100 μl of RNase-Free Water.
Figure 1: Electrophoresis plot of RNA extracted from crucian carp.
