RNase A
RNase A is derived from bovine pancreas, which is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.
RNase A
RNase A is derived from bovine pancreas, which is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide. The resulting 2′, 3′-cyclic phosphate is hydrolyzed to the corresponding 3′-nucleoside phosphate. Unhybridized RNA regions in DNA:RNA or RNA:RNA hybrids can be removed; It can be used to determine the location of a single-base mutation in DNA or RNA, annealing the labeled RNA probe with the DNA or RNA containing the single-base substitution to be examined, and the mismatched bases in the RNA:RNA or RNA:DNA can be cleaved by RNase A. It is widely used to remove RNA from DNA samples.
Price List
| Cat. No. | specification | Price |
| 8001001 | 1 ml | contact us |
Introduction
RNase A is derived from bovine pancreas, which is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide. The resulting 2′, 3′-cyclic phosphate is hydrolyzed to the corresponding 3′-nucleoside phosphate. Unhybridized RNA regions in DNA:RNA or RNA:RNA hybrids can be removed; It can be used to determine the location of a single-base mutation in DNA or RNA, annealing the labeled RNA probe with the DNA or RNA containing the single-base substitution to be examined, and the mismatched bases in the RNA:RNA or RNA:DNA can be cleaved by RNase A. It is widely used to remove RNA from DNA samples.
Experimental data
Figure 1: Plasmid DNA extracted from the same fresh bacteria cultured using the Simgen Rapid Plasmid DNA Extraction Mini Kit.
1/2：without adding RNase A. 3/4：adding RNase A.
M：Simgen DL2000 Ladder.
