500 bp DNA Ladder
500 bp DNA Ladder is composed of 8 individual DNA fragments, presenting 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 500 bp sharp bands respectively. 500 bp DNA Ladder contains 1×Loading Buffer, users can apply 5 – 10 μl in agarose gel electrophoresis directly.
Price List
Product No | Quantity | Price |
MD1003 | 250 µl | contact us |
MD1103 | 1.25ml | contact us |
INTRODUCTION
500 bp DNA Ladder is composed of 8 individual DNA fragments, presenting 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 500 bp sharp bands respectively. 500 bp DNA Ladder contains 1×Loading Buffer, users can apply 5 – 10 μl in agarose gel electrophoresis directly.
The red and yellow tracking dye in 500 bp DNA Ladder will not weakened the DNA bands under UV light, better than bromophenol blue and xylene cyanol FF.
PRECAUTION
- Clear bands can be obtained by applying 5 μl DNA Ladder when the lane width is less than 5 mm. Ifthe lane is wider, loading volume of DNA Ladder should be increased
- For DNA electrophoresis, agarose purity is of great significance to DNA band definition. Therefore, agarose with good quality should be used and gel concentration of 1%– 2 % is recommended.
- During agarose electrophoresis, the concentration of agarose is closely associated with the separation of DNA High agarose concentration is ideal for the separation of the short DNA fragments. While low agarose concentration is ideal to separate the long DNA fragments.